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PubMed · 9394792

Ia expression and antigen presentation by glia: strain and cell type-specific differences among rat astrocytes and microglia.

E N Klyushnenkova·P Vanguri
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Abstract

Astrocytes from experimental allergic encephalomyelitis (EAE)-susceptible Lewis rats expressed higher levels of Interferon-gamma-inducible Ia than astrocytes from EAE-resistant Brown Norway (BN) rats, whereas BN microglia expressed higher Ia than Lewis at both mRNA and protein levels. Lewis astrocytes induced proliferation of MBP-specific T cells selected on Lewis background as efficiently as Lewis thymocytes, whereas BN astrocytes were much less efficient in stimulating T cells selected in the presence of BN thymocytes. Microglia, irrespective of strain, induced only weak proliferative responses of these T cells despite the high expression of Ia. Antigen-stimulated T cells underwent apoptosis in the presence of microglia but not astrocytes. Thus, astrocyte-mediated proliferation of MBP-specific T cells may contribute to the development of EAE, while microglia-induced T cell apoptosis may downregulate immunopathological processes in the brain.

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AnimalsAntigen-Presenting CellsAntigensAntigens, SurfaceApoptosisAstrocytesCell DivisionCells, CulturedGene Expression RegulationHistocompatibility Antigens Class IIInterferon-gammaMicrogliaMyelin Basic ProteinRatsRats, Inbred BNRats, Inbred LewSpecies SpecificityT-LymphocytesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

E N Klyushnenkova, P Vanguri. 1997. Ia expression and antigen presentation by glia: strain and cell type-specific differences among rat astrocytes and microglia.. https://doi.org/10.1016/s0165-5728(97)00123-9

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