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PubMed · 9402283

A simple and objective approach to identifying human round spermatids.

T Angelopoulos·L Krey·A McCullough·A Adler·J A Grifo
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Abstract

Although round spermatids have been studied extensively using staining techniques and electron microscopy, little information is available about their appearance in living conditions. We describe a method of collecting and identifying round spermatids from ejaculates and testicular biopsies. The validity of the selection procedure was confirmed by fluorescence in-situ hybridization. Based on cell size, morphological characteristics of nucleus and cytoplasm, and on the nucleus/cytoplasm ratio, we harvested a population of cells that was 84% haploid. This procedure can be applied to select spermatids for clinical or research purposes.

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BiopsyCell NucleusCell SeparationCentrifugation, Density GradientCytoplasmHaploidyHumansIn Situ Hybridization, FluorescenceMaleMicroscopySpermatidsStaining and LabelingTestisJournal Article

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T Angelopoulos, L Krey, A McCullough, A Adler, J A Grifo. 1997. A simple and objective approach to identifying human round spermatids.. https://doi.org/10.1093/humrep%2F12.10.2208

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