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HPLC/circular dichroism detection using a split-type flow cell for polarized photometric detector.

A Yamamoto·A Matsunaga·K Hayakawa·M Nishimura
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Abstract

HPLC/circular dichroism detection using a conventional spectrophotometric detector has been established. A split-type flow cell developed for a polarized photometric detector, which is a non-modulated polarimeter, was inserted into this detector. When two rays of circularly polarized light with opposite rotational senses to each other were incident in both cells by combining a polarizer and a 1/4 wave retarder in front of the cell assembly, the output from this spectrophotometric detector became solely the signal based on circular dichroism. Selective detection of a chiral analyte, in which the ratio of the difference in the absorbance between two rays mentioned above and the absorbance by normal rays in over 1/1000, is feasible using the proposed instrument.

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Chromatography, High Pressure LiquidCircular DichroismSensitivity and SpecificitySpectrophotometry, UltravioletJournal Article

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A Yamamoto, A Matsunaga, K Hayakawa, M Nishimura. HPLC/circular dichroism detection using a split-type flow cell for polarized photometric detector.. https://doi.org/10.1002/(sici)1099-0801(199711)11%3A6%3C362%3A%3Aaid-bmc693%3E3.0.co%3B2-0

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