Nuclear transfer and reprogramming.

T Kono

doi:10.1530/ror.0.0020074

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PubMed · 9414468

Nuclear transfer and reprogramming.

T Kono

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Abstract

Nuclear transfer techniques for mammalian embryos have been developed in the last decade. Embryonic nuclei from advanced stages of preimplantation development can be fully reprogrammed and the totipotency is restored when nuclei are transferred into ooplasts. Transfer of nuclei after gene expression from the embryonic genome has started does not appear to restrict the reprogramming of these nuclei. The principles of nuclear transfer are outlined with respect to nuclear remodelling, nucleocytoplasmic interactions and effects of the cell cycle. However, the molecular mechanisms involved in reprogramming donor nuclei remain unknown. It is proposed that epigenetic DNA modification, such as DNA methylation that regulates gene expression, is related to the reprogramming of transplanted nuclei.

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Animals Cell Cycle Cell Nucleus Cloning, Organism DNA Embryo, Mammalian Embryo, Nonmammalian Embryonic and Fetal Development Female Gene Expression Regulation Genome Genotype Male Nuclear Transfer Techniques Oocytes Reproduction, Asexual Journal Article Review

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T Kono. 1997. Nuclear transfer and reprogramming.. https://doi.org/10.1530/ror.0.0020074

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