PubMed · 9418162
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L Butini. Cellular immunity.. https://pubmed.ncbi.nlm.nih.gov/9418162/
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Cardiomyopathy associated with HIV-1 infection is a well-recognized complication. However, it is unknown whether direct cardiomyocyte infection is involved in the pathogenesis of the cardiomyopathy. An HIV-1-based lentiviral vector and wild-type HIV-1 were used to infect human fetal cardiac myocytes in a primary culture. Quantitative polymerase chain reaction, viral p24 antigen determination, and immunofluorescence were used to detect the synthesis of HIV-1 DNA and proteins after the infection. High-efficiency infection occurred using the HIV-1-based lentiviral vector, although no infection occurred with the wild-type HIV-1 strain. Dual-labeling immunofluorescence for HIV-1 proteins and myosin confirmed that cardiomyocytes were infected. This in vitro analysis suggests that direct myocyte infection with wild-type HIV-1 may not be involved in the pathogenesis of HIV-1 cardiomyopathy. However, HIV-1-based vectors may prove useful for ex vivo cardiovascular gene therapy.
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A mailed questionnaire was carried out in the city of Copenhagen in which the leaders of 106 youth centres expressed their opinion on different publications concerning drug abuse and HIV/AIDS. The knowledge of the publications among the leaders was less than expected. The last known publication was recognized by 7% of the leaders, the most well known by 55%. A considerable part of the leaders stated that they had never received the publications. In centres where the leaders knew of the publications they were widely used, most often in centres for older members. A group of leaders stated, that the publications were irrelevant for members aged 9-14 years. The study points out specific problems in the health educations process: The publication should aim more specifically at the group and at subpopulations in the group. The distribution should be secured and the knowledge of the supply of health education material among the leaders of the youth centres should be raised.