Directed evolution of enzyme catalysts.

O Kuchner; F H Arnold

doi:10.1016/s0167-7799(97)01138-4

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Directed evolution of enzyme catalysts.

O Kuchner·F H Arnold

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Abstract

Directed enzyme evolution has emerged in the past few years as a powerful alternative to rational approaches for engineering biocatalysts. Prerequisites for successful directed evolution are functional expression in a suitable microbial host, a rapid screen for the desired feature(s) and a well-thought-out working strategy for navigating protein landscapes. The rapidly growing body of literature on enzyme evolution in vitro includes techniques for creating and searching combinatorial enzyme libraries, as well as several successful examples of different evolutionary strategies being used.

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Biotechnology Enzymes Evolution, Molecular Mutagenesis Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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O Kuchner, F H Arnold. 1997. Directed evolution of enzyme catalysts.. https://doi.org/10.1016/s0167-7799(97)01138-4

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