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Quantification of apoptotic cells using isolated glomeruli.

S Omori·T Morioka·M Wakasugi·H Kawachi·M Arakawa·F Shimizu·T Oite
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Abstract

In order to detect apoptotic cells in the kidney, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method is used in tissue sections. But the number of apoptotic cells per glomerulus in several experimental models of nephritides is about 1 per single tissue section even at peak levels. In this study, we have reported that the TUNEL method and immunostaining of cell-specific markers to a whole isolated glomerulus in combination with laser scan microscopy are potentially useful methods for the analysis of cell turnover within glomeruli.

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AnimalsApoptosisCell CountDNA FragmentationDNA NucleotidylexotransferaseDeoxyuracil NucleotidesKidney GlomerulusMacrophages, PeritonealRatsRats, WistarRenal CirculationJournal ArticleResearch Support, Non-U.S. Gov't

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S Omori, T Morioka, M Wakasugi, H Kawachi, M Arakawa, F Shimizu, T Oite. 1997. Quantification of apoptotic cells using isolated glomeruli.. https://doi.org/10.1159/000190327

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