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PubMed · 9459331

Rapid detection of D-dimer using a fiber optic biosensor.

C A Rowe·J S Bolitho·A Jane·P G Bundesen·D B Rylatt·P R Eisenberg·F S Ligler
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Abstract

We describe a rapid and sensitive method for detection and quantification of D-dimer and other crosslinked fibrin degradation products (XL-FDPs), which are present in elevated concentrations in patients with sepsis and thrombotic disorders. The method utilizes a sandwich fluoroimmunoassay immobilized in the sensing region of an evanescent wave biosensor. Physiological concentrations of D-dimer and high molecular weight XL-FDP could be determined in buffer and plasma samples on calibrated fibers in 11 min. Samples from septic patients were assayed using ELISA and the fiber optic method; concentrations determined by fiber optic assay were strongly correlated with those determined by ELISA (r = 0.918); intra- and inter-assay errors were comparable to those from ELISAs. Given its accuracy and rapid response time, this fiber optic biosensor shows great potential for development as a diagnostic tool.

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Biosensing TechniquesBuffersEnzyme-Linked Immunosorbent AssayFemaleFiber Optic TechnologyFibrin Fibrinogen Degradation ProductsHumansMaleMolecular WeightOptical FibersReference StandardsReference ValuesReproducibility of ResultsSensitivity and SpecificityTime FactorsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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C A Rowe, J S Bolitho, A Jane, P G Bundesen, D B Rylatt, P R Eisenberg, F S Ligler. 1998. Rapid detection of D-dimer using a fiber optic biosensor.. https://pubmed.ncbi.nlm.nih.gov/9459331/

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