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Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing.

L C Waters·S C Jacobson·N Kroutchinina·J Khandurina·R S Foote·J M Ramsey
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Abstract

The steps of cell lysis, multiplex PCR amplification, and electrophoretic analysis are executed sequentially on a monolithic microchip device. The entire microchip is thermally cycled to lyse cells and to amplify DNA, and the products are then analyzed using a sieving medium for size separation and an intercalating dye for fluorescence detection. Using a standard PCR protocol, a 500-base pair (bp) region of bacteriophage lambda DNA and 154-, 264-, 346-, 410-, and 550-bp regions of E. coli genomic and plasmid DNAs are amplified. The electrophoretic analysis of the products is executed in <3 min following amplification using hydroxyethyl cellulose or poly(dimethylacrylamide) sieving gels. Product sizing is demonstrated by proportioning the amplified product with a DNA sizing ladder.

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DNA, BacterialElectrophoresis, Polyacrylamide GelEscherichia coliMicrocomputersPolymerase Chain ReactionJournal Article

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L C Waters, S C Jacobson, N Kroutchinina, J Khandurina, R S Foote, J M Ramsey. 1998-01-01. Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing.. https://doi.org/10.1021/ac970642d

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