Study of erythromycin A decomposition products in aqueous solution by solid-phase microextraction/liquid chromatography/tandem mass spectrometry.

D A Volmer; J P Hui

doi:10.1002/(sici)1097-0231(19980214)12:3<123::aid-rcm126>3.0.co;2-4

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Study of erythromycin A decomposition products in aqueous solution by solid-phase microextraction/liquid chromatography/tandem mass spectrometry.

D A Volmer·J P Hui

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Abstract

The decomposition of erythromycin A (EA) in aqueous solution was examined in the pH range 2-13 by means of combined solid-phase microextraction (SPME) and liquid chromatography/electrospray ionization tandem mass spectrometry. Degradation of EA, especially at lower pH values (pH < or = 3), was very rapid and yielded a wide variety of decomposition products. Identification of these degradation products was achieved by means of tandem mass spectrometry in the product ion and precursor ion scan modes. Anhydroerythromycin A was shown to be the major reaction product in both acidic and basic solutions. Among the different SPME fibers investigated for extraction from aqueous solutions, polydimethylsiloxane/divinylbenzene exhibited the best performance for EA and its degradation products.

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D A Volmer, J P Hui. 1998. Study of erythromycin A decomposition products in aqueous solution by solid-phase microextraction/liquid chromatography/tandem mass spectrometry.. https://doi.org/10.1002/(sici)1097-0231(19980214)12%3A3%3C123%3A%3Aaid-rcm126%3E3.0.co%3B2-4

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