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A microbore high performance liquid chromatography/electrospray ionization mass spectrometry method for the determination of the phytoestrogens genistein and daidzein in comminuted baby foods and soya flour.

K A Barnes·R A Smith·K Williams·A P Damant·M J Shepherd
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Abstract

A microbore high performance liquid chromatographic/electrospray/mass spectrometric (HPLC/ESI-MS) method has been developed for the determination of the phytoestrogens daidzein and genistein in soya flours and baby foods. The samples were hydrolysed and extracted with acetonitrile-water prior to analysis. LC was performed on a microbore Primesphere 5C8 column using a water/acetonitrile/acetic acid mobile phase at a flow rate of 60 microliters/min. Atmospheric pressure ionization in the form of pneumatically assisted electrospray mass spectrometry (ESI-MS) was used as the method of detection. The limit of detection was 0.2 mg/ kg for daidzein and 0.7 mg/kg for genistein in the flour and food samples. The method proved both robust and reliable when operated over a long time period (10 days, 463 injections) generating precision data with a coefficient of variation of 4-15%.

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CalibrationChromatography, High Pressure LiquidEstrogens, Non-SteroidalFlourFood ContaminationGenisteinHydrolysisInfant FoodIsoflavonesMass SpectrometryQuality ControlReference StandardsGlycine maxJournal Article

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BibTeXRIS

K A Barnes, R A Smith, K Williams, A P Damant, M J Shepherd. 1998. A microbore high performance liquid chromatography/electrospray ionization mass spectrometry method for the determination of the phytoestrogens genistein and daidzein in comminuted baby foods and soya flour.. https://doi.org/10.1002/(sici)1097-0231(19980214)12%3A3%3C130%3A%3Aaid-rcm127%3E3.0.co%3B2-9

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