Cleaving DNA with DNA.

N Carmi; S R Balkhi; R R Breaker

doi:10.1073/pnas.95.5.2233

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PubMed · 9482868

Cleaving DNA with DNA.

N Carmi·S R Balkhi·R R Breaker

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Abstract

A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This "deoxyribozyme" can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and triplex interactions to bind and cleave separate DNA substrates. Bimolecular deoxyribozyme-mediated strand scission proceeds with a kobs of 0.2 min-1, whereas the corresponding uncatalyzed reaction could not be detected. The duplex and triplex recognition domains can be altered, making possible the targeted cleavage of single-stranded DNAs with different nucleotide sequences. Several small synthetic DNAs were made to function as simple "restriction enzymes" for the site-specific cleavage of single-stranded DNA.

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Base Sequence Catalysis DNA DNA Primers DNA, Single-Stranded Kinetics Molecular Sequence Data Nucleic Acid Conformation Polymerase Chain Reaction Substrate Specificity Journal Article Research Support, Non-U.S. Gov't

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N Carmi, S R Balkhi, R R Breaker. 1998-03-03. Cleaving DNA with DNA.. https://doi.org/10.1073/pnas.95.5.2233

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