Altered antigen presentation in mice lacking H2-O.

M Liljedahl; O Winqvist; C D Surh; P Wong; K Ngo; L Teyton; P A Peterson; A Brunmark; A Y Rudensky; W P Fung-Leung; L Karlsson

doi:10.1016/s1074-7613(00)80475-6

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PubMed · 9492004

Altered antigen presentation in mice lacking H2-O.

M Liljedahl·O Winqvist·C D Surh·P Wong·K Ngo·L Teyton·P A Peterson·A Brunmark·A Y Rudensky·W P Fung-Leung·L Karlsson

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Abstract

HLA-DM catalyzes the release of MHC class II-associated invariant chain-derived peptides (CLIP) from class II molecules. Recent evidence has suggested that HLA-DO is a negative regulator of HLA-DM in B cells, but the physiological function of HLA-DO remains unclear. Analysis of antigen presentation by B cells from mice lacking H2-O (the mouse equivalent of HLA-DO), together with biochemical analysis using purified HLA-DO and HLA-DM molecules, suggests that HLA-DO/H2-O influences the peptide loading of class II molecules by limiting the pH range in which HLA-DM is active. This effect may serve to decrease the presentation of antigens internalized by fluid-phase endocytosis, thus concentrating the B cell-mediated antigen presentation to antigens internalized by membrane immunoglobulin.

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Animals Antigen Presentation Antigen-Presenting Cells Antigens, Differentiation, B-Lymphocyte B-Lymphocytes Fluorescent Antibody Technique Gene Targeting HLA-D Antigens Histocompatibility Antigens Class II Hydrogen-Ion Concentration Immunoglobulin G Lymph Nodes Mice Mice, Mutant Strains Peptides Precipitin Tests Spleen Journal Article

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M Liljedahl, O Winqvist, C D Surh, P Wong, K Ngo, L Teyton, P A Peterson, A Brunmark, A Y Rudensky, W P Fung-Leung, L Karlsson. 1998. Altered antigen presentation in mice lacking H2-O.. https://doi.org/10.1016/s1074-7613(00)80475-6

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