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PubMed · 9497922

High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining.

T Xie·S L Ho·O C Ma
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Abstract

Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.

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Catechol O-MethyltransferaseElectrophoresis, Polyacrylamide GelEthidiumFluorescent DyesFormamidesHumansPolymorphism, Single-Stranded ConformationalProtein DenaturationStaining and LabelingTumor Suppressor Protein p53Journal Article

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T Xie, S L Ho, O C Ma. 1997. High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining.. https://doi.org/10.1136/mp.50.5.276

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Catechol O-Methyltransferase↗
PubMed2001

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Catechol O-Methyltransferase↗