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PubMed · 9501018

Mapping of ezrin dimerization using yeast two-hybrid screening.

S G Bhartur·J R Goldenring
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Abstract

Ezrin, a membrane-cytoskeleton linker protein, is involved in the recruitment of H+/K(+)-ATPase-containing tubulovesicles to the canalicular membrane during acid secretion in the parietal cell. Ezrin exists as monomers and head-to-tail dimers in vivo, and oligomerization is presumably important for activation. In this study, we mapped regions of ezrin-ezrin interaction using the yeast two-hybrid assay. We observed that the N-terminal 283 amino acids are sufficient for interaction with the carboxyl terminal 140 amino acids. The region 333.446 inhibits this association. However, the inclusion of amino acids 283-310 appears to release the inhibition. These specific interactions may play a critical role in the formation of dimerization-competent ezrin molecules.

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AnimalsCytoskeletal ProteinsDNA-Binding ProteinsDimerizationGene Transfer TechniquesHistidineHumansParietal Cells, GastricPhosphoproteinsRabbitsRecombinant Fusion ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence HomologyTranscription Factorsbeta-GalactosidaseEzrinJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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S G Bhartur, J R Goldenring. 1998-02-24. Mapping of ezrin dimerization using yeast two-hybrid screening.. https://doi.org/10.1006/bbrc.1998.8196

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