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PubMed · 95020

Purification of mouse interferon by sequential chromatography.

I A Braude·E De Clercq
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Abstract

Two schemes for the purification of mouse interferon are described, based on the concerted application of various physicochemical and affinity/adsorption column chromatographic techniques. Mouse interferon was purified to a final specific activity of 1.0--8.0 x 10(8) units/mg when first precipitated with ammonium sulphate and further processed by hydrophobic chromatography and adsorption chromatography on AFFI-Gel 202 and Controlled Pore Glass. It was purified to a final specific activity of 2.5--3.7 x 10(8) units/mg when first precipitated with ammonium sulphate and further processed by gel filtration with Ultrogel AcA 54, ion-exchange chromatography with Carboxymethyl Boi-Gel Agarose, hydrophobic chromatography with AFFI-Gel 202 and adsorption chromatography with Controlled Pore Glass.

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AdsorptionAnimalsChromatography, AffinityChromatography, GelChromatography, Ion ExchangeElectrophoresis, Polyacrylamide GelGlassInterferonsMiceMolecular WeightJournal Article

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I A Braude, E De Clercq. 1979-04-21. Purification of mouse interferon by sequential chromatography.. https://doi.org/10.1016/s0021-9673(00)90956-7

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