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PubMed · 9512097

Improving the efficiency of antisense and EGS methods.

R Salavati·S Altman
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Abstract

Hammerhead ribozymes and stable stem loop structures function as inhibitors of 3'-5'-exonuclease degradation of external guide sequences (EGSs) when covalently linked to the 3'-end of EGS RNAs. This observation may be of use in improving the efficiency of gene inactivation techniques that use single-stranded RNA (e.g., antisense RNA, EGS RNA) in vivo.

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Base SequenceExodeoxyribonuclease VExodeoxyribonucleasesGene Expression RegulationMolecular Sequence DataNucleic Acid ConformationRNA, AntisenseRNA, CatalyticRNA, Small UntranslatedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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R Salavati, S Altman. 1998. Improving the efficiency of antisense and EGS methods.. https://doi.org/10.1089/oli.1.1998.8.63

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