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PubMed · 9515722

A rapid method for the preparation of single-cell suspensions from solid tissues.

N P Singh
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Abstract

A simple and rapid methodology for the preparation of single-cell suspension from animal tissues is described. It uses a device called a "tissue press" to mince the tissue into small pieces of desired sizes which are then dissociated into single cells using a pipette. The device is inexpensive and the method economizes time and labor. An example of its use in DNA double-strand breaks study, using microgel electrophoresis, is described.

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AnimalsBrainCell SeparationDNADNA DamageElectrophoresis, Agar GelMaleRatsRats, Sprague-DawleyJournal Article

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BibTeXRIS

N P Singh. 1998-03-01. A rapid method for the preparation of single-cell suspensions from solid tissues.. https://doi.org/10.1002/(sici)1097-0320(19980301)31%3A3%3C229%3A%3Aaid-cyto10%3E3.0.co%3B2-t

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