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Cytogenetic chromosomal preparations using 2-methoxyestradiol.

H Attalla·S Knuutila·T P Mäkelä·L C Andersson·H Adlercreutz
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Abstract

The antimitotic drug 2-methoxyestradiol is an end metabolite of catechol estrogens. In vivo, it arrests cells in mitosis by interfering with the dynamics of the mitotic spindle without disrupting tubulin formation. It has a mitotic index similar to that of Colcemid in different cell lines. Here we report that 2-methoxyestradiol can be used for making cytogenetic preparations of comparable quality to that of colcemid. In addition, 2-methoxyestradiol is devoid of the toxicity associated with Colcemid, which may make 2-methoxyestradiol useful in slowly growing samples often found in primary solid tumor cultures where a sufficient number of mitotic cells is difficult to obtain.

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2-MethoxyestradiolBone Marrow CellsCells, CulturedChromosome BandingDemecolcineEstradiolFibroblastsHumansKaryotypingLeukocytesMitosisJournal ArticleResearch Support, Non-U.S. Gov't

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H Attalla, S Knuutila, T P Mäkelä, L C Andersson, H Adlercreutz. 1998-04-15. Cytogenetic chromosomal preparations using 2-methoxyestradiol.. https://doi.org/10.1016/s0165-4608(97)00341-5

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