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A specific, nonproliferative role for E2F-5 in choroid plexus function revealed by gene targeting.

G J Lindeman·L Dagnino·S Gaubatz·Y Xu·R T Bronson·H B Warren·D M Livingston
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Abstract

Homozygous E2F-5 knockout embryos and mice have been generated. Although embryonic development appeared normal, newborn mice developed nonobstructive hydrocephalus, suggesting excessive cerebrospinal fluid (CSF) production. Although the CSF-producing choroid plexus displayed normal cellular organization, it contained abundant electron-lucent epithelial cells, consistent with excessive CSF secretory activity. Moreover, E2F-5 CNS expression in normal animals was largely confined to the choroid plexus. Cell cycle kinetics were not perturbed in homozygous knockout embryo fibroblasts. Thus, E2F-5 is not essential for cell proliferation. Rather, it affects the secretory behavior of a differentiated neural tissue.

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AllelesAnimalsCell DivisionChoroid PlexusE2F5 Transcription FactorGene DeletionGene TargetingHumansHydrocephalusMiceMice, KnockoutTranscription FactorsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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G J Lindeman, L Dagnino, S Gaubatz, Y Xu, R T Bronson, H B Warren, D M Livingston. 1998-04-15. A specific, nonproliferative role for E2F-5 in choroid plexus function revealed by gene targeting.. https://doi.org/10.1101/gad.12.8.1092

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