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PubMed · 9565358

Up-regulated expression of decay-accelerating factor (CD55) confers increased complement resistance to sprouting neural cells.

K Z Zhang·S Junnikkala·M G Erlander·H Guo·J A Westberg·S Meri·L C Andersson
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Abstract

We studied gene expression in relation to induced neural differentiation in a human neural crest-derived cell line, Paju. Messenger RNA isolated before and after treatment with phorbol 12-myristate 13-acetate was analyzed by differential display reverse transcription PCR. A strongly up-regulated expression of decay-accelerating factor (DAF, CD55) was found to parallel the induced neural sprouting while the expression of two other complement regulatory proteins (CD59/protectin, CD46/membrane cofactor protein) remained unaltered during neural differentiation. The increased membrane expression of DAF, which was also seen on neural processes and growth cones, conferred elevated resistance to complement-mediated lysis. Our findings suggest that in sprouting neurons DAF expression is up-regulated to provide additional complement resistance to pathfinding axons/dendrites invading new environment. It is also suggested that membrane expression of DAF may constitute a marker of growing and regenerating neurons.

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CD55 AntigensCarcinogensCell DifferentiationCell LineGene Expression Regulation, DevelopmentalHumansNeural CrestNeuronsTetradecanoylphorbol AcetateUp-RegulationJournal ArticleResearch Support, Non-U.S. Gov't

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K Z Zhang, S Junnikkala, M G Erlander, H Guo, J A Westberg, S Meri, L C Andersson. 1998. Up-regulated expression of decay-accelerating factor (CD55) confers increased complement resistance to sprouting neural cells.. https://doi.org/10.1002/(sici)1521-4141(199804)28%3A04%3C1189%3A%3Aaid-immu1189%3E3.0.co%3B2-d

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