Positional cloning and gene identification.

T Boehm

doi:10.1006/meth.1997.0574

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Positional cloning and gene identification.

T Boehm

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Abstract

After genetic mapping and physical representation of a particular genomic region containing the gene underlying a particular Mendelian trait, a successful positional cloning strategy depends on the efficient detection and analysis of genes in the critical interval. Several gene detection strategies are presently available to compile an inventory of genes from large genomic regions. Here, the principle of these methods is briefly reviewed and their relative value for positional cloning projects compared.

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Animals Chromosome Mapping Cloning, Molecular Conserved Sequence CpG Islands DNA DNA, Complementary Exons Mice Mutation Sequence Analysis, DNA Transcription, Genetic Journal Article

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T Boehm. 1998. Positional cloning and gene identification.. https://doi.org/10.1006/meth.1997.0574

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