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PubMed · 9584894

Technical note: improved DNA extraction from ancient bones using silica-based spin columns.

D Y Yang·B Eng·J S Waye·J C Dudar·S R Saunders
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Abstract

We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.

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AnimalsBone and BonesDNAFossilsHumansPolymerase Chain ReactionSilicon DioxideSpecimen HandlingJournal ArticleResearch Support, Non-U.S. Gov't

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D Y Yang, B Eng, J S Waye, J C Dudar, S R Saunders. 1998. Technical note: improved DNA extraction from ancient bones using silica-based spin columns.. https://doi.org/10.1002/(sici)1096-8644(199804)105%3A4%3C539%3A%3Aaid-ajpa10%3E3.0.co%3B2-1

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