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PubMed · 9586086

Affinity capillary electrophoresis using DNA conjugates.

Y Ozaki·T Ihara·Y Katayama·M Maeda
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Abstract

An oligonucleotide ((dT)12)-immobilized capillary was prepared for the affinity capillary electrophoresis of DNA. Oligonucleotides ((dA)6 and (dT)6) which had the same chain length but had different base composition were separated in high resolution in the presence of Mg2+ ion. Whereas (dA)6 eluted slightly earlier than (dT)6 in a conventional polyacrylamide-coated capillary, the order of their mobilities was reversed in the (dT)12-modified system, suggesting the complementary hydrogen bondings between (dA)6 and the immobilized (dT)12.

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Base CompositionBase SequenceDNAElectrophoresis, CapillaryElectrophoresis, Polyacrylamide GelHydrogen BondingMagnesium ChlorideOligodeoxyribonucleotidesComparative StudyJournal Article

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Y Ozaki, T Ihara, Y Katayama, M Maeda. 1997. Affinity capillary electrophoresis using DNA conjugates.. https://pubmed.ncbi.nlm.nih.gov/9586086/

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Base Composition↗
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Application of CE for determination of DNA base composition.

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Base Composition↗