A cost-effective plasmid purification protocol suitable for fluorescent automated DNA sequencing.

O U Beg; R G Holt

doi:10.1007/bf02752700

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A cost-effective plasmid purification protocol suitable for fluorescent automated DNA sequencing.

O U Beg·R G Holt

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Abstract

A cost-effective, reliable, and reproducible method has been developed to produce good-quality, double-stranded plasmid DNA for automated sequence analysis. The method incorporates modifications to a previously described plasmid-purification protocol used in manual sequencing. The quality of the DNA produced from the present protocol is suitable for automated fluorescent sequencing. Using a dye-terminator sequencing protocol, most runs using plasmid DNA prepared using this protocol produced over 700 bases with greater than 99% base-calling accuracy.

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Animals Automation Cost-Benefit Analysis Fluorescent Dyes Plasmids Sequence Analysis, DNA Trypanosoma brucei brucei Journal Article Research Support, U.S. Gov't, P.H.S.

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O U Beg, R G Holt. 1998. A cost-effective plasmid purification protocol suitable for fluorescent automated DNA sequencing.. https://doi.org/10.1007/bf02752700

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