Endothelin-1 inhibits pacemaker currents in rabbit SA node cells.

H Tanaka; Y Habuchi; M Nishio; T Yamamoto; F Suto; M Yoshimura

doi:10.1097/00005344-199800001-00126

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Endothelin-1 inhibits pacemaker currents in rabbit SA node cells.

H Tanaka·Y Habuchi·M Nishio·T Yamamoto·F Suto·M Yoshimura

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Abstract

Our recent study demonstrated that endothelin-1 (ET-1) inhibits the pacemaker activity of sinoatrial (SA) node cells via changes in the L-type Ca2+, delayed K+, and background K+ currents. Using the whole-cell patch-clamp technique in the same preparation, we found that ET-1 reduces other pacemaker currents, the T-type Ca2+ current (ICa,T) and the hyperpolarization-activated inward current (I(f)). The inhibitory actions of ET-1 on these currents were concentration-dependent, i.e., EC50 of 0.9 nM for ICa,T and 2.3 nM for I(f), with little reversal after washout of the peptide. In the presence of BQ485, both currents were not affected by ET-1. These results indicate additional mechanisms underlying negative chronotropic actions of ET-1 on the rabbit SA node.

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Animals Azepines Biological Clocks Calcium Channels Endothelin-1 Heart Rate In Vitro Techniques Membrane Potentials Oligopeptides Patch-Clamp Techniques Potassium Channels Rabbits Sinoatrial Node Journal Article

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H Tanaka, Y Habuchi, M Nishio, T Yamamoto, F Suto, M Yoshimura. 1998. Endothelin-1 inhibits pacemaker currents in rabbit SA node cells.. https://doi.org/10.1097/00005344-199800001-00126

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