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Amperometric immunosensor for lactate dehydrogenase LD-1.

S Kelly·D Compagnone·G Guilbault
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Abstract

An amperometric immunosensor for the detection of the lactate dehydrogenase isoenzyme LD-1 has been developed. Polyclonal antibodies for LD-1 have been covalently immobilised onto a preactivated Immunodyne ABC membrane, reacted with standard LD-1 solutions and placed onto a platinum working electrode polarised at +600 mV vs. Ag/AgCl. Lactate dehydrogenase activity has been measured by detection of the oxidation of NADH at the electrode surface. A calibration curve for LDH in the 0.005-0.12 U range has been obtained with a repeatability of 3% and a reproducibility of 10%. The probe exhibited good selectivity (response of LD-2 was 18% of LD-1) which was further improved using thermal treatment of the membranes. Measurement of LD-1 content in human control serum with the developed procedure gave a LD-1 concentration in the reported assigned range.

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AnimalsBiosensing TechniquesElectrochemistryHumansImmunoassayL-Lactate DehydrogenaseJournal ArticleResearch Support, Non-U.S. Gov't

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S Kelly, D Compagnone, G Guilbault. 1998-02-01. Amperometric immunosensor for lactate dehydrogenase LD-1.. https://doi.org/10.1016/s0956-5663(97)00105-x

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