Maize polyamine oxidase: primary structure from protein and cDNA sequencing.

P Tavladoraki; M E Schininà; F Cecconi; S Di Agostino; F Manera; G Rea; P Mariottini; R Federico; R Angelini

doi:10.1016/s0014-5793(98)00311-1

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Maize polyamine oxidase: primary structure from protein and cDNA sequencing.

P Tavladoraki·M E Schininà·F Cecconi·S Di Agostino·F Manera·G Rea·P Mariottini·R Federico·R Angelini

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Abstract

The first complete amino acid sequence of a flavin-containing polyamine oxidase was solved by a combined approach of nucleotide and peptide sequence analysis. A cDNA of 1737 bp, isolated from maize seedlings by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends strategies, was cloned and its sequence determined. This cDNA contains information for a polypeptide chain of 500 amino acids. Its amino-terminal sequence shows the typical features of secretion signal peptides. The primary structure of the mature protein was independently confirmed by extensive amino acid sequencing. Structural relationships with flavin-containing monoamine oxidases are also discussed.

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Amino Acid Sequence Base Sequence DNA, Complementary Molecular Sequence Data Monoamine Oxidase Oxidoreductases Acting on CH-NH Group Donors Plant Proteins Sequence Alignment Sequence Homology, Amino Acid Zea mays Polyamine Oxidase Journal Article Research Support, Non-U.S. Gov't

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P Tavladoraki, M E Schininà, F Cecconi, S Di Agostino, F Manera, G Rea, P Mariottini, R Federico, R Angelini. 1998-04-10. Maize polyamine oxidase: primary structure from protein and cDNA sequencing.. https://doi.org/10.1016/s0014-5793(98)00311-1

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