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PubMed · 9603174

CD30 expression identifies a functional alloreactive human T-lymphocyte subset.

O M Martinez·J Villanueva·S Abtahi·P R Beatty·C O Esquivel·S M Krams
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Abstract

BACKGROUND: CD30 is a member of the tumor necrosis factor/nerve growth factor receptor family and has been proposed as a marker of specific cytokine-producing subsets in humans. Previous studies have examined the expression of CD30 on established T helper type 1 and T helper type 2 cell clones and the function of CD30+ cells after mitogenic stimulation. In this study, we examined the development and function of CD30+ T cells generated in response to alloantigen. METHODS: Primary one-way mixed lymphocyte reactions were established, and the expression of CD30 on T lymphocytes was determined by immunofluorescence and flow cytometry. Fluorescence-activated cell sorting was utilized to define the cytokine profile of alloactivated CD30+ cells after restimulation with anti-CD3 monoclonal antibodies or alloantigen. The effect of cyclosporine on the development of CD30+ cells, and on cytokines produced by CD30+ T lymphocytes, in response to alloantigen was determined. RESULTS: CD30+ T lymphocytes could be detected on day 2 of mixed lymphocyte reactions and continued to increase in number and proportion through day 6. Both CD4 and CD8 T cells expressed CD30 after primary alloantigenic stimulation. CD30+ T cells are a subset of alloactivated T cells and are the major source of interferon-gamma and interleukin-5 produced in response to alloantigen. Cyclosporine partially, but not completely, inhibits the development of CD30+ cells, and has a greater effect on interferon-gamma production than on interleukin-5 production. CONCLUSIONS: CD30+ T lymphocytes may constitute an important immunoregulatory subset in human allograft rejection.

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CD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCyclosporineCytokinesHumansImmunosuppressive AgentsInterferon-gammaInterleukin-2Interleukin-5IsoantigensKi-1 AntigenKineticsT-Lymphocyte SubsetsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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O M Martinez, J Villanueva, S Abtahi, P R Beatty, C O Esquivel, S M Krams. 1998-05-15. CD30 expression identifies a functional alloreactive human T-lymphocyte subset.. https://doi.org/10.1097/00007890-199805150-00016

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