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PubMed · 9607266

A quick method to extract DNA from paraffin-embedded tissues.

C K Tseng·N M Tsang·S C Kao·S Y Chen·Y P Chen
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Abstract

BACKGROUND: The amplification power of the polymerase chain reaction (PCR) technique has had great impact in molecular analysis, and DNA extraction is a common requirement in retrospective studies utilizing PCR as a tool. Conventional methods used in deparaffinization, harvesting and purification of DNA from paraffin-embedded tissue are time consuming and cause a significant loss in the yield of DNA. METHODS: We utilized heating-melting-cooling removal of paraffin, digestion of the sample with proteinase K and purification of the extracted DNA by a microconcentrator. The products, after PCR amplification of the p53 gene exon 8, were used to make a comparison between our method and the conventional xylene-phenol-choloform method. RESULTS: The amplified products from our method were superior to that of the conventional method. CONCLUSION: The method we propose has a better recovery of DNA and is more time efficient.

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DNAHumansParaffinPolymerase Chain ReactionTissue EmbeddingJournal ArticleResearch Support, Non-U.S. Gov't

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C K Tseng, N M Tsang, S C Kao, S Y Chen, Y P Chen. 1998. A quick method to extract DNA from paraffin-embedded tissues.. https://pubmed.ncbi.nlm.nih.gov/9607266/

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