Controlling gene expression using synthetic ligands.

V M Rivera

doi:10.1006/meth.1998.0596

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PubMed · 9608512

Controlling gene expression using synthetic ligands.

V M Rivera

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Abstract

Chemical inducers of dimerization (dimerizers) are a new class of reagents used for controlling protein-protein interactions. Use of a small-molecule, cell-permeable dimerizer to control the interaction of a transcription activation domain and a DNA-binding domain allows the level of expression of a target gene to be regulated by the concentration of dimerizer. The modular design of components has facilitated the development of multiple systems that can be used to readily generate cell lines in which basal expression of the target gene is low and induced to high levels by a nontoxic dimerizer. The development and use of one such system to control the expression of a gene in cultured cells are described.

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Cell Line Cinnamates Clone Cells Cloning, Molecular Dimerization Gene Expression Regulation Gene Targeting Genetic Vectors Humans Hygromycin B Ligands Plasmids Transcription Factors Transfection Journal Article Review

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V M Rivera. 1998. Controlling gene expression using synthetic ligands.. https://doi.org/10.1006/meth.1998.0596

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