PubMed · 9623966
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S Aamodt. 1998. Playing with dopamine release.. https://doi.org/10.1038/nm0698-660
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The transplantation of fetal mesencephalic dopaminergic tissue into the striatum of parkinsonian (PD) patients is an experimental therapeutic approach. The target population for this treatment are advanced, predominantly akinetic-rigid patients with therapy-associated complications, and not tremor-dominant PD patients. Based on a review of selected published data, no specific beneficial effect of fetal mesencephalic transplantation in PD patients was reported on the cardinal symptom rest tremor. On the one hand, this might be the result of a lack of efficacy of this treatment with respect to tremor; on the other hand, PD patients with predominant tremor may have a better prognosis with respect to motor function and therefore might not reach the advanced akinetic-rigid stage, which makes them eligible for neural transplantation. Rest-tremor dominant PD patients have not undergone fetal cell grafting; therefore its effect on rest tremor is unknown.
In situ hybridization histochemistry is widely used to study gene expression at the mRNA level in tissues and cells. Double label in situ hybridization allows for coexpression studies. We describe a protocol for the simultaneous hybridization of two cRNA probes tagged with and digoxigenin-UTP, respectively, to frozen brain tissue sections. Hybridization signals of digoxigenin-tagged probes appear as purple cytoplasmic staining following detection of digoxigenin residues by an alkaline-phosphatase-(AP)-linked antibody. Signals resulting from hybridization of radiolabeled probes are detected as silver grains overlying cellular profiles in sections coated with autoradiographic emulsion. Grain counting allows for semiquantitatively estimates of the cellular expression levels of transcripts. Suitable cRNA-probes can be derived from linear templates generated by polymerase chain reaction (PCR) using nested primers which contain RNA-polymerase promotor sites. The cRNA-probes are sensitive and allow an application of this protocol to the detection of a wide range of mRNAs of medium or low abundance.