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A miniaturized monolayer trough with variable surface area in the square-millimeter range.

C Duschl·M Boncheva·H Vogel
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Abstract

A new simple concept for a miniaturized monolayer trough is described. The overall monolayer area in the expanded state is approximately 150 mm2 and can be reduced by a factor of 2. The surface area is a function of the shape of the meniscus formed by the subphase and is controlled by the amount of water in the monolayer trough. The controlled compression of monolayers to a desired area per molecule with simultaneous observation of the lateral distribution of fluorescently labeled molecules is shown. A biological reaction between a specific antibody and lipid anchored peptide demonstrates the feasibility of monolayer experiments, which require only very small quantities of substance (in the pmol range). This trough might also be a valuable tool for the 2D crystallization of proteins at lipid layers via specific binding sites such as metal chelators.

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1,2-Dipalmitoylphosphatidylcholine4-Chloro-7-nitrobenzofurazanCapsidCapsid ProteinsLipoproteinsMembrane LipidsMicroscopy, FluorescencePolytetrafluoroethyleneSurface PropertiesViral ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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C Duschl, M Boncheva, H Vogel. 1998-05-28. A miniaturized monolayer trough with variable surface area in the square-millimeter range.. https://doi.org/10.1016/s0005-2736(98)00036-4

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