Mirror-image RNA that binds D-adenosine.

S Klussmann; A Nolte; R Bald; V A Erdmann; J P Fürste

doi:10.1038/nbt0996-1112

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PubMed · 9631061

Mirror-image RNA that binds D-adenosine.

S Klussmann·A Nolte·R Bald·V A Erdmann·J P Fürste

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Abstract

A 58-mer L-RNA ligand that binds to naturally occurring D-adenosine with a dissociation constant of 1.7 microM in solution was identified from a combinatorial library employing mirror-design. The corresponding D-RNA ligand shows identical binding affinity to L-adenosine. Reciprocal chiral specificity was also evident from ligand discrimination; the binding affinity of the L-RNA ligand for D-adenosine was 9000-fold greater than its affinity for L-adenosine and vice versa. While the D-RNA ligand was rapidly degraded in human serum, the L-RNA ligand displayed an extraordinary stability. This indicates the potential application of specifically designed L-RNA ligands as stable monoclonal antibody analogues and the development of highly stable L-ribozymes.

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Adenosine Base Sequence Binding Sites Binding, Competitive Biotechnology Consensus Sequence Conserved Sequence DNA Drug Stability Humans In Vitro Techniques Ligands Molecular Sequence Data Nucleic Acid Conformation RNA Sequence Homology, Nucleic Acid Stereoisomerism Journal Article Research Support, Non-U.S. Gov't

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S Klussmann, A Nolte, R Bald, V A Erdmann, J P Fürste. 1996. Mirror-image RNA that binds D-adenosine.. https://doi.org/10.1038/nbt0996-1112

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