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A second-step splicing activity is conserved from yeast to human.

R M Cicarelli·A Khaouja·C Codony·R Espuny·R Eritja·M Bach-Elias
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Abstract

We describe a defective HeLa nuclear extract which is particularly deficient in step 2 of splicing reaction. With this extract we have studied the conservation of a second-step activity from yeast to human cells. We detected a S. cerevisiae second-step splicing activity that allows restoration of step 2 of the defective HeLa nuclear extract, which indicates that yeast purified fraction has a second-step activity that is conserved from yeast to human cells. The activity is a yeast UsnRNP protein(s) since it is purified with anti-trimethylguanosine by immunoaffinity columns.

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Biological EvolutionChromatography, AffinityGenetic Complementation TestGlobinsHeLa CellsHumansRNA PrecursorsRNA SplicingRibonucleoproteins, Small NuclearSaccharomyces cerevisiaeJournal ArticleResearch Support, Non-U.S. Gov't

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R M Cicarelli, A Khaouja, C Codony, R Espuny, R Eritja, M Bach-Elias. 1998-06-18. A second-step splicing activity is conserved from yeast to human.. https://doi.org/10.1006/bbrc.1998.8771

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