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PubMed · 9647236

A lambda 3' enhancer drives active and untemplated somatic hypermutation of a lambda 1 transgene.

Q Kong·L Zhao·S Subbaiah·N Maizels
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Abstract

Somatic hypermutation is a highly regulated process that targets mutations to the rearranged Ig genes. Little is known about the cis-elements required for somatic hypermutation of the lambda light chain gene. We have studied somatic hypermutation of a rearranged lambda 1 transgene under the control of either a lambda 2-4 or kappa 3' enhancer. The mutations in the transgenes were analyzed by sequencing DNA amplified from hypermutating Peyer's patch B cells. The results indicate that the lambda 3' enhancer can drive active hypermutation of a lambda 1 transgene in Peyer's patch cells. The lambda 1 transgene under analysis carried two marked V lambda 2 genes immediately upstream that could serve as sequence donors in possible gene conversion events. There was no evidence of sequence transfer to the hypermutated lambda 1 gene, suggesting that gene conversion is not a major mechanism for somatic hypermutation in mice.

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AnimalsB-LymphocytesBase SequenceCell LineDNA Mutational AnalysisEnhancer Elements, GeneticGene ConversionGene Expression RegulationImmunoglobulin kappa-ChainsImmunoglobulin lambda-ChainsMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicMolecular Sequence DataMutagenesis, Site-DirectedPeyer's PatchesTemplates, GeneticTransgenesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Q Kong, L Zhao, S Subbaiah, N Maizels. 1998-07-01. A lambda 3' enhancer drives active and untemplated somatic hypermutation of a lambda 1 transgene.. https://pubmed.ncbi.nlm.nih.gov/9647236/

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