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PubMed · 9649635

Solid-phase cDNA library construction, a versatile approach.

T Roeder
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Abstract

A rapid and versatile method for cDNA library construction was developed. It is based on conventional cDNA library synthesis including all enzymatic steps usually required, but is performed on a solid support. The cDNA is immobilised via a biotin residue to streptavidin coupled magnetic beads, which allows rapid and easy to perform changes of buffers and enzymes. Therefore, it combines speed (library construction within a single day) with high quality libraries, making it ideally suited for most purposes.

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Base SequenceDNA, ComplementaryGene LibraryGenetic VectorsJournal ArticleResearch Support, Non-U.S. Gov't

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T Roeder. 1998-07-15. Solid-phase cDNA library construction, a versatile approach.. https://doi.org/10.1093/nar%2F26.14.3451

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