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PubMed · 9651253

Visualization of specific B and T lymphocyte interactions in the lymph node.

P Garside·E Ingulli·R R Merica·J G Johnson·R J Noelle·M K Jenkins
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Abstract

Early events in the humoral immune response were visualized in lymph nodes by simultaneous tracking of antigen-specific CD4 T and B cells after immunization. The T cells were initially activated in the T cell areas when the B cells were still randomly dispersed in the B cell-rich follicles. Both populations then migrated to the edges of the follicles and interacted there, resulting in CD154-dependent B cell proliferation and germinal center formation. These results provide visual documentation of cognate T-B cell interactions and localize them to the follicular border.

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Adoptive TransferAnimalsAntibody FormationAntigen PresentationB-LymphocytesCD4-Positive T-LymphocytesCD40 LigandCell MovementDendritic CellsGerminal CenterImmunizationImmunoglobulin MLymph NodesLymphocyte ActivationMembrane GlycoproteinsMiceMice, Inbred BALB CMice, Inbred C57BLMice, TransgenicPlasma CellsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P Garside, E Ingulli, R R Merica, J G Johnson, R J Noelle, M K Jenkins. 1998-07-03. Visualization of specific B and T lymphocyte interactions in the lymph node.. https://doi.org/10.1126/science.281.5373.96

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