A simple one-dimensional solid-state NMR method to characterize the nuclear spin interaction tensors associated with the peptide bond.

D K Lee; A Ramamoorthy

doi:10.1006/jmre.1998.1442

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A simple one-dimensional solid-state NMR method to characterize the nuclear spin interaction tensors associated with the peptide bond.

D K Lee·A Ramamoorthy

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Abstract

We propose a simple one-dimensional RF pulse sequence for the study of chemical shift and heteronuclear dipolar coupling tensors of oriented as well as unoriented solids. An off-resonance RF decoupling of protons during the signal acquisition of less sensitive nuclei is used to suppress homonuclear 1H-1H dipolar interactions. This method is experimentally demonstrated on peptide samples selectively labeled with 15N isotope.

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Algorithms Electron Spin Resonance Spectroscopy Hydrogen Hydrogen Bonding Magnetic Resonance Spectroscopy Magnetics Nitrogen Isotopes Peptides Protons Radio Waves Signal Processing, Computer-Assisted Valine Journal Article Research Support, Non-U.S. Gov't

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D K Lee, A Ramamoorthy. 1998. A simple one-dimensional solid-state NMR method to characterize the nuclear spin interaction tensors associated with the peptide bond.. https://doi.org/10.1006/jmre.1998.1442

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