New assay technologies for high-throughput screening.

L Silverman; R Campbell; J R Broach

doi:10.1016/s1367-5931(98)80015-x

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New assay technologies for high-throughput screening.

L Silverman·R Campbell·J R Broach

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Abstract

The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to interference from test compounds needed to function in a high-throughput screening mode. Similarly, novel cell-based assays are now being adapted for high-throughput screening, providing for in situ analysis of a variety of biological targets. Finally, recent advances in assay miniaturization mark a transition to ultra high-throughput screening, ensuring that identification of lead compounds will not be the rate-limiting step in finding new drugs.

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Animals Cell Line Cell-Free System Chemistry, Organic Drug Design Drug Evaluation, Preclinical Energy Transfer Genes, Reporter Mammals Miniaturization Spectrometry, Fluorescence Transfection Journal Article Review

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L Silverman, R Campbell, J R Broach. 1998. New assay technologies for high-throughput screening.. https://doi.org/10.1016/s1367-5931(98)80015-x

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