A new NEDD8-ligating system for cullin-4A.

F Osaka; H Kawasaki; N Aida; M Saeki; T Chiba; S Kawashima; K Tanaka; S Kato

doi:10.1101/gad.12.15.2263

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A new NEDD8-ligating system for cullin-4A.

F Osaka·H Kawasaki·N Aida·M Saeki·T Chiba·S Kawashima·K Tanaka·S Kato

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Abstract

NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an E1 (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino- and carboxy-terminal regions of E1 and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.

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Amino Acid Sequence Animals Cell Cycle Proteins Cullin Proteins DNA-Binding Proteins Humans In Vitro Techniques Ligases Molecular Sequence Data NEDD8 Protein Rabbits Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Ubiquitin-Activating Enzymes Ubiquitin-Protein Ligases Ubiquitins Journal Article

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F Osaka, H Kawasaki, N Aida, M Saeki, T Chiba, S Kawashima, K Tanaka, S Kato. 1998-08-01. A new NEDD8-ligating system for cullin-4A.. https://doi.org/10.1101/gad.12.15.2263

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