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A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence.

H Yamada·S Ikeda-Wada·K Oguri
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Abstract

A new screening method for amphetamines was developed. It consists of derivatization with dansyl chloride, extraction of the derivative using a Sep-Pak C18 or a Bond Elut C18, solid phase extraction columns, and visualization of the fluorescence of the cartridge. A control test using drug-free urine showed no fluorescence. Amphetamine, methamphetamine and the methylenedioxy derivatives exhibited strong fluorescence, while related compounds, such as N-ethylamphetamine and fenetylline, were negative or weakly positive. The disadvantage of the present method is that it is a multi-step procedure and 20-30 min is required for screening. However, since it has a different specificity from the widely used immunochemical technique, it is suggested to be a useful screen for amphetamines.

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AmphetamineDansyl CompoundsFluorescenceHumansMethamphetamineSensitivity and SpecificitySubstance Abuse DetectionJournal ArticleResearch Support, Non-U.S. Gov't

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H Yamada, S Ikeda-Wada, K Oguri. 1998. A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence.. https://doi.org/10.1248/bpb.21.778

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