Oncogene amplification and expression in pediatric solid tumors.

J Mares; V Polanská; H Görgens; Z Sedlácek; T Maríková; P Bocek; R Kodet; J Schackert; P Goetz

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PubMed · 9717522

Oncogene amplification and expression in pediatric solid tumors.

J Mares·V Polanská·H Görgens·Z Sedlácek·T Maríková·P Bocek·R Kodet·J Schackert·P Goetz

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Abstract

Oncogene amplification and expression and their mutual relationship was analyzed in 92 pediatric tumors by Southern and Northern blot hybridization with N-MYC, ERB A, ERB B, N-RAS and Shb probes. Amplification and overexpression was associated with more advanced clinical stages of tumor, especially in neuroblastomas, rhabdomyosarcomas and ganglioneuroblastomas. The most frequent alteration observed was N-MYC amplification together with overexpression. N-RAS amplification was not detected, while the overexpression of this oncogene was found in 3 cases. Neither amplification nor overexpression was revealed in any specimen of hepatoblastoma or hepatocellular carcinoma. We suggest that oncogenes overexpression provides more accurate prognostic information than amplification.

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Child Child, Preschool Gene Amplification Gene Expression Humans Neoplasms Oncogenes Polymerase Chain Reaction Journal Article Research Support, Non-U.S. Gov't

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J Mares, V Polanská, H Görgens, Z Sedlácek, T Maríková, P Bocek, R Kodet, J Schackert, P Goetz. 1998. Oncogene amplification and expression in pediatric solid tumors.. https://pubmed.ncbi.nlm.nih.gov/9717522/

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