Possible stage-specific function of NF-kappaB during pre-B cell differentiation.

L Guerrini; A Molteni; F Blasi

doi:10.1016/s0014-5793(98)00965-x

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Possible stage-specific function of NF-kappaB during pre-B cell differentiation.

L Guerrini·A Molteni·F Blasi

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Abstract

Lipopolysaccharide (LPS)-induced differentiation of the murine pre-B cell line 70Z/3 is a model for pre-B to B cell differentiation and has been used to show that the transcription factor NF-kappaB is essential to induce the expression of the Ig kappa gene. We have investigated the mechanism involved in late stages of the process when all cells have reached a more mature B phenotype, i.e. beyond 48 up to 96 h of LPS treatment. NF-kappaB binding activity was induced at early times by LPS treatment, but its DNA binding activity disappeared after 84 h of LPS treatment. Accumulation of IkappaB alpha protein in the nucleus correlated with the disappearance of NF-kappaB activity at 72, 84 and 96 h, and treatment of nuclear extracts of 72-96 h LPS-treated cells with Na-deoxycholate restored NF-kappaB binding activity. The data indicate that NF-kappaB, while important to initiate the process of Ig kappa gene transcription in 70Z/3 pre-B cells, is no longer required for its maintenance in differentiated 70Z/3 cells.

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Animals B-Lymphocytes Cell Differentiation Cell Line Gene Expression Regulation, Developmental Hematopoietic Stem Cells Lipopolysaccharides Mice NF-kappa B Journal Article Research Support, Non-U.S. Gov't

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L Guerrini, A Molteni, F Blasi. 1998-08-28. Possible stage-specific function of NF-kappaB during pre-B cell differentiation.. https://doi.org/10.1016/s0014-5793(98)00965-x

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