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PubMed · 9740654

Centromeric protein B null mice are viable with no apparent abnormalities.

A V Perez-Castro·F L Shamanski·J J Meneses·T L Lovato·K G Vogel·R K Moyzis·R Pedersen
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Abstract

The centromere protein B (CENP-B) is a centromeric DNA/binding protein. It recognizes a 17-bp sequence motif called the CENP-B box, which is found in the centromeric region of most chromosomes. It binds DNA through its amino terminus and dimerizes through its carboxy terminus. CENP-B protein has been proposed to perform a vital role in organizing chromatin structures at centromeres. However, other evidence does not agree with this view. For example, CENP-B is found at inactive centromeres on stable dicentric chromosomes, and also mitotically stable chromosomes lacking alpha-satellite DNA have been reported. To address the biological function of CENP-B, we generated mouse null mutants of CENP-B by homologous recombination. Mice lacking CENP-B were viable and fertile, indicating that mice without CENP-B undergo normal somatic and germline development. Thus, both mitosis and meiosis are able to proceed normally in the absence of CENP-B.

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AnimalsAutoantigensCell LineCentromere Protein BChromosomal Proteins, Non-HistoneCrosses, GeneticDNA-Binding ProteinsFertilityFetal ViabilityGenotypeHistocytochemistryImmunohistochemistryLitter SizeMaleMiceMice, KnockoutMicrotubulesRNA, MessengerSperm CountTestisJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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A V Perez-Castro, F L Shamanski, J J Meneses, T L Lovato, K G Vogel, R K Moyzis, R Pedersen. 1998-09-15. Centromeric protein B null mice are viable with no apparent abnormalities.. https://doi.org/10.1006/dbio.1998.9005

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