A monocot pollen-specific promoter contains separable pollen-specific and quantitative elements.

D A Hamilton; Y H Schwarz; J P Mascarenhas

doi:10.1023/a:1006083725102

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A monocot pollen-specific promoter contains separable pollen-specific and quantitative elements.

D A Hamilton·Y H Schwarz·J P Mascarenhas

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Abstract

The region of the promoter of the pollen-specific maize gene, ZM13, from -119 to -37 was analyzed by a linker-scanning type of substitution mutagenesis and two areas were shown to be responsible for pollen expression: a proximal region delineated by mutations from -84 to -53 that conferred pollen specificity, and an upstream region delineated by a mutation from -107 to -102 (Q-element) that could increase the expression of the proximal region but showed no ability to cause expression in pollen on its own. Replacement of both of these areas with other sequences including the CaMV 35S promoter failed to replace activity.

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Allergens Antigens, Plant Arabidopsis Base Sequence DNA, Plant Gene Expression Genes, Plant Genes, Reporter Glucuronidase Mutation Plant Proteins Plants, Genetically Modified Pollen Promoter Regions, Genetic Zea mays Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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D A Hamilton, Y H Schwarz, J P Mascarenhas. 1998-11-01. A monocot pollen-specific promoter contains separable pollen-specific and quantitative elements.. https://doi.org/10.1023/a%3A1006083725102

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