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PubMed · 9751116

Basic fibroblast growth factor-induced decrease in type I collagen gene transcription is mediated by B-myb.

K E Kypreos·M A Nugent·G E Sonenshein
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Abstract

Basic fibroblast growth factor (bFGF), a member of the fibroblast growth factor family, potently induces increased vascular smooth muscle cell (SMC) proliferation and decreased expression of type I collagen. Recently, our laboratory demonstrated that, in bovine vascular SMCs, expression of B-myb, a member of the myb gene family, is dependent upon cellular growth state and that B-myb decreases alpha1(I) collagen promoter activity in transient transfection assays. Nuclear run-off analysis indicated that the decrease in alpha1(I) collagen mRNA level seen upon bFGF treatment was due to a decline in the rate of alpha1(I) procollagen gene transcription. Thus, we investigated the potential role of B-Myb in the down-regulation of type I collagen gene expression by bFGF. Using Northern blot analysis, we found that bFGF treatment of bovine aortic SMCs caused an increase in B-myb mRNA levels. Ectopic expression of B-myb decreased endogenous alpha1(I) collagen mRNA levels. Importantly, introduction of a B-myb antisense oligonucleotide prevented the drop in the alpha1(I) collagen mRNA levels seen upon treatment with bFGF. Together, these results indicate that B-myb mediates signals leading to the decreased rate of alpha1(I) collagen gene transcription caused by bFGF.

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AnimalsCattleCell Cycle ProteinsCells, CulturedCollagenDNA-Binding ProteinsDose-Response Relationship, DrugFemaleFibroblast Growth Factor 2Gene Expression RegulationGenesOligonucleotides, AntisenseProcollagenRNA, MessengerTrans-ActivatorsTranscription, GeneticJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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K E Kypreos, M A Nugent, G E Sonenshein. 1998. Basic fibroblast growth factor-induced decrease in type I collagen gene transcription is mediated by B-myb.. https://pubmed.ncbi.nlm.nih.gov/9751116/

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