Two complementary strand-specific termination sites for adenovirus DNA replication.

B Weingärtner; E L Winnacker; A Tolun; U Pettersson

doi:10.1016/0092-8674(76)90117-3

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Two complementary strand-specific termination sites for adenovirus DNA replication.

B Weingärtner·E L Winnacker·A Tolun·U Pettersson

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Abstract

Adenovirus type 2 DNA, specifically labeled at the termini for DNA replication, was prepared by isolation of viral DNA molecules which were completed during short pulses with 3H-thymidine. The distribution of radioactivity in the two complementary strands at the termini for DNA replication was determined by liquid phase hybridization and gelelectrophoresis. At the right-hand terminus, nearly all radioactivity was found in the viral h strand, whereas at the left-hand terminus, most radioactivity was confined to the viral l strand. The results suggest that both molecular ends serve as origins and termini for replication of adenovirus type 2 DNA.

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Adenoviridae DNA Replication DNA Restriction Enzymes DNA, Viral Electrophoresis Models, Biological Nucleic Acid Hybridization Nucleic Acid Renaturation Thymidine Virus Replication Journal Article

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B Weingärtner, E L Winnacker, A Tolun, U Pettersson. 1976. Two complementary strand-specific termination sites for adenovirus DNA replication.. https://doi.org/10.1016/0092-8674(76)90117-3

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