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PubMed · 9762095

Protoheme extraction from plant tissue.

L C Stillman·M L Gassman
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Abstract

A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether. After evaporation of the ether, the residue is dissolved in alkaline pyridine, and the protoheme concentration is estimated from a dithionite-reduced-minus-ferricyanide-oxidized spectrum. When compared to some other methods, this procedure gives consistently higher yields.

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AcetoneEtherHemeIndicators and ReagentsPlantsPyridinesReproducibility of ResultsSpectrophotometryTemperatureComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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L C Stillman, M L Gassman. 1978. Protoheme extraction from plant tissue.. https://doi.org/10.1016/0003-2697(78)90827-8

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