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PubMed · 9769731

Chimeras and mosaics in mouse mutant analysis.

J Rossant·A Spence
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Abstract

As the number of mouse mutants generated by gene targeting continues to grow exponentially, the challenge is not how to generate a mutant but how to analyse the phenotype. Genes might play multiple roles in development and act in cell-autonomous and cell non-autonomous modes, making phenotypic analysis complex. Genetic mosaic analysis is a powerful tool for dissecting complex gene functions. Classical preimplantation chimeras made between mutant and wild-type embryos can answer many questions, and new genetic techniques for generating restricted genetic mosaicism promise to enhance the future power of mosaic analysis in mammals.

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AnimalsChimeraGene TargetingMiceMice, Mutant StrainsMosaicismMutationPhenotypeJournal ArticleReview

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J Rossant, A Spence. 1998. Chimeras and mosaics in mouse mutant analysis.. https://doi.org/10.1016/s0168-9525(98)01552-2

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